Composite

Part:BBa_K642007:Design

Designed by: Ian Roney, Matthew Orton   Group: iGEM11_uOttawaCA   (2011-10-24)

Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 89
    Illegal BsaI.rc site found at 1379


Design Notes

The yeGFP part (BBa_K319039) from last year didn't have a stop codon so this tagged promoter should have a terminator sequence cloned downstream before it is used. We defined the distal region as the most upstream 516 nucleotides of the Adh1 promoter and the proximal region as the most downstream 207 nucleotides of the Gal1 promoter.

Source

The distal region of the Adh1 promoter was PCR amplified from a plasmid source of BBa_K319005. The Gal1 promoter is a commonly used promoter in our lab and we amplified it from a plasmid source. yeGFP was PCR amplified from a plasmid source of BBa_K319039.

References